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Rabbit Anti-ERK1 + ERK2 antibody (bs-0022R)
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说明书:     
20ul/380.00元
50ul/780.00元
100ul/1380.00元
200ul/2200.00元
大包装/询价

产品编号 bs-0022R
英文名称 ERK1 + ERK2
中文名称 丝裂原活化蛋白激酶1/ERK 1/2抗体
别    名 ERK 1/2; ERK 1; ERK 2; ERK-2; ERK1; ERK2; ERT1; ERT2; Extracellular signal regulated kinase 1; Extracellular signal regulated kinase 2; Extracellular signal-regulated kinase 2; HS44KDAP; HUMKER1A; Insulin stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; MAPK1; MAPK2; MAPK3; MGC20180; Microtubule associated protein 2 kinase; Mitogen activated protein kinase 1; Mitogen activated protein kinase 2; Mitogen activated protein kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; MK01_HUMAN; MK03_HUMAN; p38; p40; p41; p41mapk; p42 MAPK; p42-MAPK; p42MAPK; p44 ERK1; p44 MAPK; p44ERK1; p44MAPK; PRKM 1; PRKM 2; PRKM 3; PRKM1; PRKM2; PRKM3; Protein kinase mitogen activated 1; Protein kinase mitogen activated 2; Protein kinase mitogen activated 3; Protein tyrosine kinase ERK 2.  
Specific References  (5)     |     bs-0022R has been referenced in 5 publications.
[IF=3.03] Sun, Jing, et al. "Hypoglycemic effect and mechanism of honokiol on type 2 diabetic mice." Drug Design, Development and Therapy 9 (2015): 6327.  WB ;  Mouse.  
[IF=1.58] Sun, Jing, et al. "Magnolia officinalis extract contains potent inhibitors against PTP1B and attenuates hyperglycemia in db/db mice." BioMed Research International 2015 (2015).  WB ;  Mouse.  
[IF=2.27] Yu, Wu, et al. "BEX4 upregulation alters Sertoli cell growth properties and protein expression profiles: An explanation for cadmium‐induced testicular Sertoli cell injury." Journal of Biochemical and Molecular Toxicology (2017).  
[IF=3.86] Chu, Meiqiang, et al. "MicroRNA-126 participates in lipid metabolism in mammary epithelial cells." Molecular and Cellular Endocrinology (2017).  WB ;  Human.  
[IF=3.22] Du, Wei, et al. "Pinellia ternata Attenuates Mucus Secretion and Airway Inflammation after Inhaled Corticosteroid Withdrawal in COPD Rats." The American Journal of Chinese Medicine 44.05 (2016): 1027-1041.  WB ;  Rat.  
规格价钱 50ul/780元     100ul/1380元     200ul/2200元     大包装/询价
说 明 书     
研讨范畴                     
抗体泉源 Rabbit
克隆范例 Polyclonal
交织回响反映 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, 
产物运用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 42kDa
细胞定位 细胞核 细胞浆 细胞膜 细胞中基质 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 本 KLH conjugated synthetic peptide derived from human ERK2:301-358/358 
亚    型 IgG
纯化要领 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
生存前提 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed
产物引见 background:
The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation,differentiation, and cell cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed. [provided by RefSeq, Jul 2008].

Function:
Serine/threonine kinase which acts as an essentialcomponent of the MAP kinase signal transduction pathway. MAPK1/ERK2and MAPK3/ERK1 are the 2 MAPKs which play an important role in theMAPK/ERK cascade. They participate also in a signaling cascadeinitiated by activated KIT and KITLG/SCF. Depending on the cellularcontext, the MAPK/ERK cascade mediates diverse biological functionssuch as cell growth, adhesion, survival and differentiation throughthe regulation of transcription, translation, cytoskeletalrearrangements. The MAPK/ERK cascade plays also a role ininitiation and regulation of meiosis, mitosis, and postmitoticfunctions in differentiated cells by phosphorylating a number oftranscription factors. About 160 substrates have already beendiscovered for ERKs. Many of these substrates are localized in thenucleus, and seem to participate in the regulation of transcriptionupon stimulation. However, other substrates are found in thecytosol as well as in other cellular organelles, and those areresponsible for processes such as translation, mitosis andapoptosis. Moreover, the MAPK/ERK cascade is also involved in theregulation of the endosomal dynamics, including lysosome processingand endosome cycling through the perinuclear recycling compartment(PNRC); as well as in the fragmentation of the Golgi apparatusduring mitosis. The substrates include transcription factors (suchas ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements(such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1),regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3,MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and avariety of other signaling-related molecules (like ARHGEF2, DCC,FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1,RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1,MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) andphosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are othersubstrates which enable the propagation the MAPK/ERK signal toadditional cytosolic and nuclear targets, thereby extending thespecificity of the cascade. Mediates phosphorylation of TPR inrespons to EGF stimulation. May play a role in the spindle assemblycheckpoint. Phosphorylates PML and promotes its interaction withPIN1, leading to PML degradation (By similarity).
Acts as a transcriptional repressor. Binds to a[GC]AAA[GC] consensus sequence. Repress the expression ofinterferon gamma-induced genes. Seems to bind to the promoter ofCCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 andSTAT1. Transcriptional activity is independent of kinase activity.

Subunit:
Binds both upstream activators and downstream substratesin multimolecular complexes. Interacts with ADAM15, ARHGEF2, ARRB2,DAPK1 (via death domain), HSF4, IER3, IPO7, DUSP6, NISCH, SGK1, andisoform 1 of NEK2. Interacts (via phosphorylated form) with TPR(via C-terminus region and phosphorylated form); the interactionrequires dimerization of MAPK1/ERK2 and increases following EGFstimulation. Interacts (phosphorylated form) withCAV2 ('Tyr-19'-phosphorylated form); the interaction, promoted byinsulin, leads to nuclear location and MAPK1 activation. Interacts with DCC. Interacts withMORG1, PEA15 and MKNK2. MKNK2 isoform 1 binding prevents fromdephosphorylation and inactivation. The phosphorylated forminteracts with PML.

Subcellular Location:
Cytoplasm, cytoskeleton, spindle. Nucleus. Cytoplasm, cytoskeleton, centrosome. Cytoplasm. Note=Associated with the spindle duringprometaphase and metaphase. PEA15-binding andphosphorylated DAPK1 promote its cytoplasmic retention.Phosphorylation at Ser-244 and Ser-246 as well asautophosphorylation at Thr-188 promote nuclear localization.

Tissue Specificity:
Widely expressed.

Post-translational modifications:
Dually phosphorylated on Thr-183 and Tyr-185, which activatesthe enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-185. Phosphorylated upon FLT3 and KIT signaling.

Similarity:
Belongs to the protein kinase superfamily. CMGCSer/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.

SWISS:
P27361

Gene ID:
5595

Database links:

Human

Human

Mouse

Mouse

Rat

Rat

Cow

Human

Human

Cow

Human

Human

Mouse

Mouse

Rat

Rat

Human

Human

Mouse

Mouse

Rat

Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

激酶和磷酸酶(Kinases and Phosphatases)
丝裂原活化蛋白激酶-ERK(Mitogen-activated protein kinase 1, MAPK-1)是一组能够被多种细胞中旌旗灯号即得到卵白丝/苏氨酸激酶,处于胞浆旌旗灯号传导通路的末终位置,活化后转位到核内,做用于核内转录因子,调治基因表达。它重要到场生长因子、激素、细胞因子、应激等种种刺激下细胞的回响反映、细胞的发展、分化历程。
卵白分子量:42kDa。
经研讨证明,MAPK旌旗灯号转导通路存在于大多数细胞内,正在将细胞中刺激旌旗灯号转导至细胞及其核内,并引发细胞生物学回响反映(如细胞增殖、分化、转化及凋亡等)的历程中具有至关重要的感化。研讨注解,MAPK旌旗灯号转导通路正在细胞内具有生物退化的高度守旧性,正在低等原核细胞和高档哺乳类细胞内,现在均已发明存在着多条并行的MAPK旌旗灯号通路,差别的细胞中刺激可运用差别的MAPK旌旗灯号通路,经由过程其互相调控而介导差别的细胞生物学回响反映。
产品图片
Sample:
Brain (Rat) Lysate at 30 ug
Heart (Rat) lysate at 30 ug
Primary: Anti- ERK2/MAPK1 (bs-0022R) at 1/200 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample: Placenta(Mouse) Lysate at 40 ug
Primary: Anti- ERK1+ERK2 (bs-0022R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample: MCF-7 Cell Lysate at 40 ug
Primary: Anti- ERK1+ERK2 (bs-0022R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 42 kD
Observed band size: 43 kD
Sample: Lovo Cell Lysate at 30 ug
Primary: Anti-ERK1+ERK2 (bs-0022R) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 42 kD
Observed band size: 41 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ERK1 + ERK2) Polyclonal Antibody, Unconjugated (bs-0022R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ERK2/MAPK1 Polyclonal Antibody, Unconjugated(bs-0022R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hep G2 cells (blue).
Primary Antibody:Rabbit Anti-ERK1 + ERK2 antibody(bs-0022R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (bs-0022R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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