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Rabbit Anti-GLUT4 antibody (bs-0384R)
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说明书:     
20ul/380.00元
50ul/780.00元
100ul/1380.00元
200ul/2200.00元
大包装/询价

产品编号 bs-0384R
英文名称 GLUT4
中文名称 葡萄糖转运卵白4抗体
别    名 insulin-responsive; Glucose transporter GLUT 4; Glucose Transporter GLUT4; Glucose transporter type 4; Glucose transporter type 4 insulin responsive; GLUT 4; GLUT-4; GLUT4; GTR4_HUMAN; kug; SLC 2A4; SLC2A4; solute carrier family 2 (facilitated glucose transporter) member 4; Solute carrier family 2, facilitated glucose transporter member 4.  
规格价钱 50ul/780元     100ul/1380元     200ul/2200元     大包装/询价
说 明 书     
研讨范畴             
抗体泉源 Rabbit
克隆范例 Polyclonal
交织回响反映 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, 
产物运用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 54kDa
细胞定位 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 本 KLH conjugated synthetic peptide derived from human GLUT4:401-509/509 <Cytoplasmic>
亚    型 IgG
纯化要领 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
生存前提 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed
产物引见 background:
GLUT4 is the facilitated glucose transporter expressed exclusively in adipocytes and muscle cells, and is also known as the "insulin-responsive" glucose transporter. GLUT4 translocates from an ill-defined intracellular compartment to the plasma membrane in response to insulin. The total cellular content of GLUT4 is significantly decreased in adipose cells from many patients with Type II diabetes mellitus, and animals with some types of experimental diabetes.

Function:
Insulin-regulated facilitative glucose transporter.

Subcellular Location:
Endomembrane system. Cytoplasm > perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.

Tissue Specificity:
Skeletal and cardiac muscles; brown and white fat.

Post-translational modifications:
Sumoylated.

DISEASE:
Defects in SLC2A4 may be a cause of noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. Defects in SLC2A4 may be a cause of certain post-receptor defects in NIDDM. The variant in position Ile-383 is found in a small number of NIDDM patients, but seems not to be found in nondiabetic subjects.

Similarity:
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

SWISS:
P14672

Gene ID:
6517

Database links:

 Cow

 Human

 Mouse

 Rat

 Human

 Cow

 Cow

 Human

 Mouse

 Rat

 Human

 Mouse

 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

葡萄糖转运卵白-4是一种十分重要的葡萄糖转运体,取胰岛素反抗和2型糖尿病亲切相干.葡萄糖转运卵白4正在细胞内部和细胞膜之间轮回活动.实现对葡萄糖的转运需求葡萄糖转运卵白4本身的转位和活化.葡萄糖转运卵白4取瘦削、肿瘤相干联.
GLUT-2和GLUT-4卵白那两个葡萄糖运载体的研讨关于糖尿病的胰岛素开释停滞和胰岛素反抗有重要意义.
产品图片
Sample:
Heart(Rat) Cell Lysate at 40 ug
Primary: Anti-GLUT4 (bs-0384R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Sample:
muscle (mouse) Lysate at 40 ug
heart (mouse) Lysate at 40 ug
Primary: Anti- beta tubulin(bs-0384R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 54 kD
www.9822.com
Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti- GLUT4 (bs-0384R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated(bs-0384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated(bs-0384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): K562 (blue).
Primary Antibody (green line): Rabbit Anti-GLUT4 antibody (bs-0384R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (overnight at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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